3D Artificial Skin Used To Treat Spina Bifida In Rats – Asian Scientist Magazine
By LizaAVILA
Scientists have used artificial skin grafts grown from induced pluripotent stem cells to cover the developing spines of rat fetuses while still in the womb.
Asian Scientist Newsroom | June 14, 2017 | In the Lab
AsianScientist (Jun. 14, 2017) - Researchers from Japan have developed a stem cell-based therapy to treat a severe congenital bone defect known as myelomeningocele. Their findings have been published in Stem Cell Reports.
Myelomeningocele is the most serious and common form of spina bifida, a condition in which the backbone and spinal canal do not close before birth, leaving parts of the spinal cord and nerves exposed.
A baby born with this disorder typically has an open area or a fluid-filled sac on the mid to lower back. Most children with this condition are at risk of brain damage because too much fluid builds up in their brains. They also often experience symptoms such as loss of bladder or bowel control, loss of feeling in the legs or feet, and paralysis of the legs.
To develop a minimally invasive treatment to cover large myelomeningocele defects earlier during pregnancy, the researchers first generated artificial skin from human induced pluripotent stem cells (iPSCs), and then successfully transplanted the skin grafts into rat fetuses with the condition.
We provide preclinical proof of concept for a fetal therapy that could improve outcomes and prevent lifelong complications associated with myelomeningoceleone of the most severe birth defects, said senior study author Professor Akihiro Umezawa of Japan's National Research Institute for Child Health and Development.
Since our fetal cell treatment is minimally invasive, it has the potential to become a much-needed novel treatment for myelomeningocele.
The human iPSCs were generated from fetal cells taken from amniotic fluid from two pregnancies with severe fetal disease (Down syndrome and twin-twin transfusion syndrome). The researchers then used a chemical cocktail in a novel protocol to turn the iPSCs into skin cells and treated these cells with additional compounds such as epidermal growth factor to promote their growth into multi-layered skin.
In total, it took approximately 14 weeks from amniotic fluid preparation to 3D skin generation, which would allow for transplantation to be performed in humans during the therapeutic window of 28-29 weeks of gestation.
Next, the researchers transplanted the 3D skin grafts into 20 rat fetuses through a small incision in the uterine wall. The artificial skin partially covered the myelomeningocele defects in eight of the newborn rats and completely covered the defects in four of the newborn rats, protecting the spinal cord from direct exposure to harmful chemicals in the external environment.
Moreover, the engrafted 3D skin regenerated with the growth of the fetus and accelerated skin coverage throughout the pregnancy period. Notably, the transplanted skin cells did not lead to tumor formation, but the treatment significantly decreased birth weight and body length.
We are encouraged by our results and believe that our fetal stem cell therapy has great potential to become a novel treatment for myelomeningocele, Umezawa said. However, additional studies in larger animals are needed to demonstrate that our fetal stem cell therapy safely promotes long-term skin regeneration and neurological improvement.
The article can be found at: Kajiwara et al. (2017) Fetal Therapy Model of Myelomeningocele with Three-dimensional Skin Using Amniotic Fluid Cell-derived Induced Pluripotent Stem Cells.
Source: Cell Press; Photo: Kazuhiro Kajiwara. Disclaimer: This article does not necessarily reflect the views of AsianScientist or its staff.
The rest is here:
3D Artificial Skin Used To Treat Spina Bifida In Rats - Asian Scientist Magazine
- 001 Ying Liu discusses IPS cell therapy for ALS [Last Updated On: August 6th, 2011] [Originally Added On: August 6th, 2011]
- 002 Jeanne Loring talks about stem cells, part 2 [Last Updated On: September 5th, 2011] [Originally Added On: September 5th, 2011]
- 003 Embryonic Stem Cells From Skin: Making Old Cells Young [Last Updated On: September 5th, 2011] [Originally Added On: September 5th, 2011]
- 004 IPs cells Part3 [Last Updated On: September 6th, 2011] [Originally Added On: September 6th, 2011]
- 005 IPs cells Part 2 [Last Updated On: September 7th, 2011] [Originally Added On: September 7th, 2011]
- 006 A Century of Stem Cells - Johns Hopkins Medicine [Last Updated On: September 7th, 2011] [Originally Added On: September 7th, 2011]
- 007 Stem Cell Implications for ALS (Amyotrophic Lateral Sclerosis) [Last Updated On: September 7th, 2011] [Originally Added On: September 7th, 2011]
- 008 Myelin Repair Foundation on Stem Cell Research [Last Updated On: September 9th, 2011] [Originally Added On: September 9th, 2011]
- 009 IPs Cells Part 4 [Last Updated On: September 11th, 2011] [Originally Added On: September 11th, 2011]
- 010 National Medical Report [Last Updated On: September 13th, 2011] [Originally Added On: September 13th, 2011]
- 011 IPs cells Part 1 [Last Updated On: September 15th, 2011] [Originally Added On: September 15th, 2011]
- 012 iPS Stem Cell-Based Treatment of Epidermolysis Bullosa [Last Updated On: September 16th, 2011] [Originally Added On: September 16th, 2011]
- 013 Jeanne Loring talks about stem cells, part 1 [Last Updated On: September 16th, 2011] [Originally Added On: September 16th, 2011]
- 014 Kristopher Nazor 2 [Last Updated On: September 16th, 2011] [Originally Added On: September 16th, 2011]
- 015 Andalusian Stem Cell Bank [Last Updated On: September 19th, 2011] [Originally Added On: September 19th, 2011]
- 016 Cellular Reprogramming Stem Cell Domain Name For Sale! - CellularReprogramming.com [Last Updated On: September 20th, 2011] [Originally Added On: September 20th, 2011]
- 017 Dr. Oz to Oprah and Michael J Fox: "The stem cell debate is dead." [Last Updated On: September 22nd, 2011] [Originally Added On: September 22nd, 2011]
- 018 Manning, Owens Try Stem Cell Therapy [Last Updated On: October 5th, 2011] [Originally Added On: October 5th, 2011]
- 019 Jeanne Loring talks about stem cells, part 3 [Last Updated On: October 5th, 2011] [Originally Added On: October 5th, 2011]
- 020 Epidermolysis Bullosa: Corrected iPS Stem Cell-Based Therapy - Video [Last Updated On: October 13th, 2011] [Originally Added On: October 13th, 2011]
- 021 Introduction to Stem Cells - Video [Last Updated On: October 14th, 2011] [Originally Added On: October 14th, 2011]
- 022 Parkinson's Disease: Progress and Promise in Stem Cell Research - Video [Last Updated On: October 14th, 2011] [Originally Added On: October 14th, 2011]
- 023 stem cell research - Video [Last Updated On: December 7th, 2011] [Originally Added On: December 7th, 2011]
- 024 Ian Wilmut discusses stem cell and direct cellular transformation therapy - Video [Last Updated On: December 8th, 2011] [Originally Added On: December 8th, 2011]
- 025 Jeff Bluestone: Immune rejection of stem cell transplants - Video [Last Updated On: December 8th, 2011] [Originally Added On: December 8th, 2011]
- 026 Advances in Stem Cell Research: Shinya Yamanaka - Video [Last Updated On: December 11th, 2011] [Originally Added On: December 11th, 2011]
- 027 2011 Summit: Stem Cells, Reprogramming and Personalized Medicine, Rudolf Jaenisch, MD - Video [Last Updated On: December 14th, 2011] [Originally Added On: December 14th, 2011]
- 028 Parkinson's Disease: Advancing Stem Cell Therapies - 2011 CIRM Grantee Meeting - Video [Last Updated On: January 20th, 2012] [Originally Added On: January 20th, 2012]
- 029 Professor Alan Trounson - World focus on stem cell research - Video [Last Updated On: January 27th, 2012] [Originally Added On: January 27th, 2012]
- 030 Stanford scientists turn skin cells into neural precusors, bypassing stem-cell stage [Last Updated On: January 31st, 2012] [Originally Added On: January 31st, 2012]
- 031 Researchers turn skin cells into neural precusors, bypassing stem-cell stage [Last Updated On: January 31st, 2012] [Originally Added On: January 31st, 2012]
- 032 “Wide-ranging applications for pluripotent stem cells” [Last Updated On: February 2nd, 2012] [Originally Added On: February 2nd, 2012]
- 033 Radiation treatment transforms breast cancer cells into cancer stem cells [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- 034 Radiation Treatment Generates Cancer Stem Cells from Less Aggressive Breast Cancer Cells [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- 035 Radiation treatment generates cancer stem cells from less aggressive breast cancer cells, study suggests [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- 036 Life Technologies Scientist Uma Lakshmipathy presents, "Solving Challenges in the Generation of Induced Pluripotent ... [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- 037 Radiation therapy transforms breast cancer cells into cancer stem cells [Last Updated On: February 14th, 2012] [Originally Added On: February 14th, 2012]
- 038 Research and Markets: Primary and Stem Cells: Gene Transfer Technologies and Applications [Last Updated On: February 15th, 2012] [Originally Added On: February 15th, 2012]
- 039 Horizon in new super-cell elite [Last Updated On: February 16th, 2012] [Originally Added On: February 16th, 2012]
- 040 Presentations at the Society of Toxicology Annual Meeting Demonstrate Superior Predictivity of Cellular Dynamics ... [Last Updated On: March 8th, 2012] [Originally Added On: March 8th, 2012]
- 041 New approach to treating type 1 diabetes? Transforming gut cells into insulin factories [Last Updated On: March 12th, 2012] [Originally Added On: March 12th, 2012]
- 042 Gut cells transformed into insulin factories 'could help to treat type I diabetes' [Last Updated On: March 12th, 2012] [Originally Added On: March 12th, 2012]
- 043 A new approach to treating type I diabetes? Gut cells transformed into insulin factories [Last Updated On: March 12th, 2012] [Originally Added On: March 12th, 2012]
- 044 Columbia Researchers Find Potential Role for Gut Cells in Treating Type I Diabetes [Last Updated On: March 12th, 2012] [Originally Added On: March 12th, 2012]
- 045 Study demonstrates cells can acquire new functions through transcriptional regulatory network [Last Updated On: March 14th, 2012] [Originally Added On: March 14th, 2012]
- 046 Gut Cells Turned To Insulin Factories - New Type l Diabetes Treatment [Last Updated On: March 14th, 2012] [Originally Added On: March 14th, 2012]
- 047 Cellular Dynamics Expands Distribution Agreement with iPS Academia Japan, Inc. to Include Distribution of iCell ... [Last Updated On: March 28th, 2012] [Originally Added On: March 28th, 2012]
- 048 :: 20, Apr 2012 :: IBN DISCOVERS HUMAN NEURAL STEM CELLS WITH TUMOR TARGETING ABILITY – A PROMISING DISCOVERY FOR ... [Last Updated On: April 22nd, 2012] [Originally Added On: April 22nd, 2012]
- 049 Human neural stem cells with tumor targeting ability discovered [Last Updated On: April 22nd, 2012] [Originally Added On: April 22nd, 2012]
- 050 IBN Discovers Human Neural Stem Cells, Promising Discovery For Breast Cancer Therapy [Last Updated On: April 22nd, 2012] [Originally Added On: April 22nd, 2012]
- 051 IBN Discovers Human Neural Stem Cells with Tumor Targeting Ability - A Promising Discovery for Breast Cancer Therapy [Last Updated On: April 22nd, 2012] [Originally Added On: April 22nd, 2012]
- 052 VistaGen Secures Key U.S. Patent Covering Stem Cell Technology Methods Used to Test Drug Candidates for Liver Toxicity [Last Updated On: April 25th, 2012] [Originally Added On: April 25th, 2012]
- 053 Improved adult-derived human stem cells have fewer genetic changes than expected [Last Updated On: May 2nd, 2012] [Originally Added On: May 2nd, 2012]
- 054 Researchers restore neuron function to brains damaged by Huntington's disease [Last Updated On: May 31st, 2012] [Originally Added On: May 31st, 2012]
- 055 Cellular Dynamics Launches MyCell™ Services [Last Updated On: June 7th, 2012] [Originally Added On: June 7th, 2012]
- 056 Fate Therapeutics And BD Biosciences Launch BD™ SMC4 To Improve Cellular Reprogramming And IPS Cell Culture Applications [Last Updated On: June 12th, 2012] [Originally Added On: June 12th, 2012]
- 057 Life Technologies and Cellular Dynamics International Partner for Global Commercialization of Novel Stem Cell ... [Last Updated On: June 13th, 2012] [Originally Added On: June 13th, 2012]
- 058 LIFE Focuses on Stem Cell Research - Analyst Blog [Last Updated On: June 20th, 2012] [Originally Added On: June 20th, 2012]
- 059 International Stem Cell Corp Granted Key Patent for Liver Disease Program [Last Updated On: September 30th, 2012] [Originally Added On: September 30th, 2012]
- 060 NeuroGeneration Recruits Top Scientist To Direct New Division of Biotherapeutics and Drug Discovery In La Jolla, CA [Last Updated On: October 5th, 2012] [Originally Added On: October 5th, 2012]
- 061 FRC Supports Alliance Defending Freedom, Jubilee Campaign Cert Petition to Supreme Court on Stem Cell Funding [Last Updated On: October 11th, 2012] [Originally Added On: October 11th, 2012]
- 062 10/11/2012 10:05 JAPAN Nobel Prize for Yamanaka, scientific research and ethics must go hand in hand [Last Updated On: October 11th, 2012] [Originally Added On: October 11th, 2012]
- 063 Read in [Last Updated On: October 12th, 2012] [Originally Added On: October 12th, 2012]
- 064 Induced pluripotent stem cell - Wikipedia, the free encyclopedia [Last Updated On: November 3rd, 2013] [Originally Added On: November 3rd, 2013]
- 065 What are induced pluripotent stem cells or iPS cells? - Stem Cells ... [Last Updated On: November 3rd, 2013] [Originally Added On: November 3rd, 2013]
- 066 Stem Cell Definitions | California's Stem Cell Agency [Last Updated On: November 3rd, 2013] [Originally Added On: November 3rd, 2013]
- 067 iPSCTherapy.com: Induced Pluripotent Stem Cell therapy Information ... [Last Updated On: November 8th, 2013] [Originally Added On: November 8th, 2013]
- 068 Human muscle stem cell therapy gets help from zebrafish [Last Updated On: November 8th, 2013] [Originally Added On: November 8th, 2013]
- 069 Induced pluripotent stem cell therapy - Wikipedia, the free ... [Last Updated On: November 8th, 2013] [Originally Added On: November 8th, 2013]
- 070 IPS Cell Therapy - Genetherapy [Last Updated On: November 8th, 2013] [Originally Added On: November 8th, 2013]
- 071 MD Supervised Stem Cell Therapy [Last Updated On: November 9th, 2013] [Originally Added On: November 9th, 2013]
- 072 Stem Cell Therapy for Neuromuscular Diseases | InTechOpen [Last Updated On: November 23rd, 2013] [Originally Added On: November 23rd, 2013]
- 073 Combining Stem Cell Therapy with Gene Therapy | Boston ... [Last Updated On: November 25th, 2013] [Originally Added On: November 25th, 2013]
- 074 Biomanufacturing center takes central role in developing stem ... [Last Updated On: December 4th, 2013] [Originally Added On: December 4th, 2013]
- 075 Stem Cell Quick Reference - Learn Genetics [Last Updated On: December 6th, 2013] [Originally Added On: December 6th, 2013]
- 076 Induced Pluripotent Stem Cells (iPS) from Human Skin: Probable ... [Last Updated On: December 6th, 2013] [Originally Added On: December 6th, 2013]
- 077 'Something positive for humankind': Girls lend cells to genetic study [Last Updated On: December 13th, 2013] [Originally Added On: December 13th, 2013]
- 078 Stem cell science: Can two girls help change the face of medicine? [Last Updated On: December 13th, 2013] [Originally Added On: December 13th, 2013]
- 079 Okyanos Heart Institute CEO Matt Feshbach Congratulates Japan’s Legislators On Stem Cell Bill And Global Regulatory ... [Last Updated On: December 13th, 2013] [Originally Added On: December 13th, 2013]
- 080 Stem cells for Parkinson's getting ready for clinic [Last Updated On: December 13th, 2013] [Originally Added On: December 13th, 2013]
