San Diego, CA (PRWEB) December 22, 2014

Stemiotics, Inc., a supplier of stem cell generation services, today announced it has licensed key intellectual property pertaining to the application of modified RNA from CELLSCRIPT, LLC of Madison, WI. CELLSCRIPT holds an exclusive license to a portfolio of issued and pending patents based on discoveries made at the University of Pennsylvania covering the use of synthetic messenger RNA (mRNA) containing modified nucleotides to evade antiviral responses in mammalian cells. This breakthrough technology has opened new vistas for the application of mRNA as a gene expression vector in human therapeutics and cell fate manipulation. The license to Stemiotics is for use of modified RNA in the production of human induced pluripotent stem cells (iPSCs) for research applications such as disease modeling and drug discovery.

Stemiotics is already using CELLSCRIPT's ultra-low immunogenicity mRNA to reprogram human skin cells into pluripotent stem cells with the potential to become any cell type in the body. In addition to the incorporation of modified nucleotides, CELLSCRIPT's advanced synthetic mRNA is subject to novel purification techniques that virtually eliminate residual innate immune responses to the mRNA on delivery into human or animal cells in vivo or in culture. Stemiotics is committed to applying clinically relevant, state-of-the-art technology in its iPSC derivation pipeline. The company uses only xeno-free reagents at all steps of the process, from the initial expansion of the donor skin cells to the cryogenic preservation of the artificially-induced pluripotent stem cells. Stemiotics employs the most potent cocktail of cellular reprogramming factors currently available, including engineered transcription factors based on IP which has been exclusively licensed to CELLSCRIPT. This sophisticated technology allows Stemiotics to convert human skin cells into pluripotent stem cells in just over a week in feeder-free conditions and without the need for drug-like small molecule accelerants.

Stemiotics believes that the mRNA-based reprogramming system it has developed is the fastest, most productive and safest approach to converting human skin cells into pluripotent stem cells yet devised. The company offers high-throughput iPSC derivation on a fee-for-service basis with fast turnaround times and at a cost of only $1000 per line, an order of magnitude below prevailing industry norms. The licensing relationship with CELLSCRIPT will further enhance Stemiotics position as an emerging leader in the field of cellular reprogramming, with all its great promise for advancing the understanding of disease, the development of new drugs and, ultimately, for cell-based therapies and regenerative medicine.

http://www.stemiotics.com

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Stemiotics Licenses Modified RNA for Cell Reprogramming

Nola, the only surviving northern white rhinoceros at the San Diego Zoo Safari Park, rests at the facility on Dec. 18. / photo by Charlie Neuman * U-T San Diego

When the northern white rhinoceros Angalifu died at the San Diego Zoo Safari Park last week, he left his species a step closer to extinction. Only five of his kind remain, most of them elderly.

However, the gentle, two-ton animal also left behind a part of himself that may let scientists breathe new life into the imperiled species. They plan to use DNA samples preserved in the San Diego Frozen Zoo to create more white rhinos.

In their most ambitious vision something that has never been tried for any creature other than lab mice the researchers aim to coax skin cells from Angalifu and others of his kind to become stem cells, and then sperm and eggs, and then implant the embryos in surrogate rhinos.

This approach would go beyond cloning by producing more genetic diversity in the resulting offspring. Its unclear how long scientists will need to achieve the unprecedented feat, but they remain committed to the years-long effort.

Its really brilliant in retrospect that when animals die, you can freeze some of their cells and theyll last forever, said Jeanne Loring, a stem cell pioneer at The Scripps Research Institute in La Jolla who is a member of the project.

Angalifu came to the San Diego Zoo in 1990, joining two females, Nola and Noti, who had arrived a year earlier. The easygoing animals were favorites with zookeepers, who enjoyed training them and scratching their thick but sensitive hides.

Northern white rhinos, which once roamed central Africa in Chad, Uganda, Sudan and the Central African Republic, have been nearly wiped out by civil war and poaching. Their horns are valued as dagger handles and are mistakenly seen as an aphrodisiac or medicinal aid.

Researchers and zoo officials in several countries decided to try to preserve the species through captive breeding of the few remaining northern white rhinoceroses.

The San Diego Zoo Safari Park had succeeded in breeding southern white rhinos, a close relative of the northern variety. Nearly 100 southern white calves have been born at the facility.

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Can scientists clone a rhinoceros?

System for marking slow-cycling SCs in vivo and monitoring their fate. (A) Strategy. (B to D) Skin sections of mice before and after 4-week chase. Shown are epifluorescence of H2B-GFP (green) and 4,6-diamidino-2-phenylindole (DAPI) (blue), and indirect immunofluorescence with antibodies (Abs) indicated (Texas Red). The hair cycle stage is indicated on each set of after chase frames (see also fig. S1, B to D, and fig. S2). Arrows (B) denote Ki67+ sebaceous gland cells in telogen. Arrowheads [(B) and (C)] denote transition zone between bulge and newly generated follicle downgrowth. Late anagen (Ki67 in red): GFP-bright cells are retained in the bulge; their progeny rapidly divide, diluting H2B-GFP. (D) Early anagen II bulb overexposed for GFP and double-labeled (small arrowheads) with Abs against each differentiation cell type. (E) Mice after chase were scratch-wounded and analyzed by immunofluorescence. Arrows denote likely directions of movements of GFP-positive LRCs and progeny. Abbreviations: Bu, bulge; DP, dermal papilla; Mx, matrix; hg, hair germ; Ep, epidermis; asterisk, hair shaft (autofluorescent); hf, hair follicle; Cx, cortex; ORS/IRS, outer/inner root sheaths; BM, basement membrane; In, infundibulum; W, wound. Scale bars, 50 m.

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Defining the epithelial stem cell niche in skin.

Manhattan NY (PRWEB) December 13, 2014

ABRING, one of the pioneers in the new era of the most advanced skin care trend, announced the debut of their two new skin care products: ABRING lemon stem cell acne serum and ABRING apple stem cell serum/eyes serum, These two new cutting-edge skin care products contain concentrated essence which is derived from Californias organic plants and has no artificial or chemical components. This condensed essence has been widely recognized for its obvious effect on anti-aging and stimulation of skin cells regeneration. As a result, ABRINGs new stem cell products not only have such distinctive functions as anti-aging, supplementing moisture, alleviating scars appearance and whitening skin, but also can be safely used by pregnant women since it only contains pure natural plant ingredients.

It is well-known that the skin is exposed to all kinds of radiations everyday which can damage our skin in various ways. Most people think that there is nothing to worry because they have already used segregation frost. However, what they dont realize is the fact that segregation frost only plays a trivial role in isolation and doesnt help repair or stimulate skin cells renewal or regeneration activities.

Stem cells are capable of self-reproducing and have lots of potentials. Under different conditions, they can evolve into various functional cells. Therefore, the activity of skin stem cells directly affects the external appearance of the skin. ABRING uses the newest stem cell research achievement and is a known brand for natural beauty products. Because it contains condensed essences concentrated from organic plants and is free of any chemicals, ABRING can stimulate activity in skin cells, slow down the aging process, increase elasticity, improve tone, and reduce the appearance of scars. In addition, because ABRING also contains a lot of mineral water and vitamin C, it can effectively improve skin brightening and help cure and prevent acne.

ABRING products founder, Albert, born in California, United, is a cell biologist and a biochemist. Unlike many, he didnt have a carefree and happy childhood as the result of a natural disaster. However, Albert wasnt defeated by the unpredicted distress. Instead, he was dedicated to study and graduated from Columbia University. In 1971, invited by the U.S. government, Doctor Albert became one of first post-war medical doctors. In same year, Doctor Albert established ABRING laboratory which stands for: Doctor Albert brings hope. Based on years of research at Columbia University focusing on stem cell biology, Doctor Albert found that certain raw materials can effectively remove skin scars without using any chemical additives. After over 7000 experiments, he finally extracted pure activating factors from natural plants that can help alleviate the appearance of scars. Dr. Albert named the condensed essence of concentration of plant stem cells as ABRING. Since then, with its innovative and effective way of enhancing the tone of skin, ABRING started to be recognized more and more by the world. Doctor Albert's efforts eventually got paid off and ABRING became one of the favored skin care products from the users of all classes. Nowadays, ABRING has been used by more than 500 famous beauty salons over the world. Moreover, the product has been widely recommended by doctors as daily lotion for skin disease treatment or post-surgery care.

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ABRING Announces Debut of Stem Cell Based Skin Care Products

TIME Health medicine This New Kind of Stem Cell May Revolutionize How We Treat Diseases Scientists have created a new type of stem cell that could speed treatments for diseases and make them safer

Ever since Japanese researcher Shinya Yamanaka found a way to treat skin cells with four genes and reprogram them back to their embryonic state, scientists have been buzzing over the promise of stem cell therapies. Stem cells can be coaxed to become any of the bodys cell types, so they could potentially replace diseased or missing cells in conditions such as diabetes or Alzheimers. And Yamanakas method also meant that these cells could be made from patients themselves, so they wouldnt trigger dangerous immune rejections.

Now scientists led by Dr. Andras Nagy at Mount Sinai Hospital Lunenfeld-Tanenbaum Research Institute in Toronto report an exciting new advance that could push stem cells even closer to the clinic. In a series of papers in the journals Nature and Nature Communications, the group describes a new class of stem cell, which they called F class, that they generated in the lab.

The F class cells, says Nagy, have a few advantages over the Yamanaka-generated induced pluripotent stem cells, or iPS cells. While the iPS cells are created by using viruses to introduce four genes that reprogram the cells, Nagys team relied on a technique they developed several years ago using transposonssmall pieces of DNA that can insert themselves into different parts of a genome. Unlike viruses, these transposons can be popped out of the genome if theyre no longer needed, and they dont carry the potential risk of viral infection.

MORE: Stem-Cell Research: The Quest Resumes

Nagys team found that the transposons were much more reliable vehicles for delivering the reprogramming genes exactly where they were needed to efficiently turn the clock back on the skin cells. Whats more, they could use the common antibiotic doxycycline to turn the four genes on and off; adding doxycycline to the cell culture would trigger the transposons to activate, thus turning on the genes, while removing the antibiotic would turn them off.

In this way, says Nagy, he was able to pump up the efficiency of the reprogramming process. Using the Yamanaka method, it was hit-or-miss whether the viruses would find their proper place in a cells genome, and more uncertainty over how effectively it could direct the cell to activate the four reprogramming genes. F class cells are much more similar [in the culture dish], like monozygotic twins while iPS cells are more like brothers and sisters, he says.

That consistency is a potential advantage of the transposon method, since any stem cell-based treatment would require a robust population of stem cells which can then be treated with the proper compounds to develop into insulin-making pancreatic cells to treat diabetes, or new nerve cells to replace dying ones in Alzheimers, or fresh heart muscle to substitute for scarred tissue after a heart attack.

MORE: Stem Cell Miracle? New Therapies May Cure Chronic Conditions like Alzheimers

Nagys team also described, with the most detail to date, exactly how mature cells like skin cells perform the ultimate molecular feat and become forever young again when exposed to the four genes. They analyzed the changes in the cells DNA, the proteins they made, and more. Its similar to high definition TV, he says. We see things much better with much more detail. We expect that having that high resolution characterization will allow us to better understand what is happening during this process at the molecular level. And obviously that better understanding is going to affect what we can do with these cells to make them better, safer and more efficient in cell-based treatments in the future.

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This New Kind of Stem Cell May Revolutionize How We Treat Diseases

Nik Spencer/Nature

Eggs and sperm do it when they combine to make an embryo. John Gurdon did it in the 1960s, when he used intestinal cells from tadpoles to generate genetically identical frogs. Ian Wilmut did it too, when he used an adult mammalian cell to make Dolly the sheep in 1996. Reprogramming reverting differentiated cells back to an embryonic state, with the extraordinary ability to create all the cells in the body has been going on for a very long time.

Scientific interest in reprogramming rocketed after 2006, when scientists showed that adult mouse cells could be reprogrammed by the introduction of just four genes, creating what they called induced pluripotent stem (iPS) cells1. The method was simple enough for almost any lab to attempt, and now it accounts for more than a thousand papers per year. The hope is that pluripotent cells could be used to repair damaged or diseased tissue something that moved closer to reality this year, when retinal cells derived from iPS cells were transplanted into a woman with eye disease, marking the first time that reprogrammed cells were transplanted into humans (see Nature http://doi.org/xhz; 2004).

There is just one hitch. No one, not even the dozen or so groups of scientists who intensively study reprogramming, knows how it happens. They understand that differentiated cells go in, and pluripotent cells come out the other end, but what happens in between is one of biology's impenetrable black boxes. We're throwing everything we've got at it, says molecular biologist Knut Woltjen of the Center for iPS Cell Research and Application at Kyoto University in Japan. It's still a really confusing process. It's very complicated, what we're doing.

Kerri Smith talks to researcher Andras Nagy and reporter David Cyranoski about reprogramming cells.

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One of the problems, stem-cell biologists say, is that their starting population contains a mix of cells, each in a slightly different molecular state. And the process for making iPS cells is currently inefficient and variable: only a tiny fraction end up fully reprogrammed and even these may differ from one another in subtle but important ways. What is more, the path to reprogramming may vary depending on the conditions under which cells are being grown, and from one lab to the next. This makes it difficult to compare experimental results, and it raises safety concerns should a mix of poorly characterized cells be used in the clinic.

But new techniques are starting to clarify the picture. By carrying out meticulous analyses of single cells and amassing reams of detailed molecular data, biologists are identifying a number of essential events that take place en route to a reprogrammed state. This week, the biggest such project an international collaboration audaciously called Project Grandiose unveiled its results26. The scientists involved used a battery of tests to take fine-scale snapshots of every stage of reprogramming and in the process, revealed an alternative state of pluripotency. It was the first high-resolution analysis of change in cell state over time, says Andras Nagy, a stem-cell biologist at Mount Sinai Hospital in Toronto, Canada, who led the project. I'm not shy about saying grandiose.

I'm not shy about saying grandiose.

But there is more to do if scientists want to control the process well enough to generate therapeutic cells with ease. Yes, we can make iPS cells and yes we can differentiate them, but I think we feel that we do not control them enough says Jacob Hanna, a stem-cell biologist at the Weizmann Institute of Science in Rehovot, Israel. Controlling cell behaviour at will is very cool. And the way to do it is to understand their molecular biology with great detail.

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Stem cells: The black box of reprogramming

Software billionaire Paul Allen says he's committing $100 million to create a new institute in Seattle focusing on the mechanics of human cell biology.

The Allen Institute for Cell Science's first project, the Allen Cell Observatory, will focus on creating computational models for the kinds of induced pluripotent stem cells, or IPS cells, that have the ability to turn into heart muscle cells or the epithelial cells that form the inner linings of organs as well as skin.

Such cells hold promise for facilitating research into how cells become diseased, and potentially for growing replacement tissues.

"Cells are the fundamental units of life, with every disease we know of affecting particular types of cells," Allen said in a news release. "Scientists have learned a great deal about many of the 50 trillion cells in our bodies over the last decades, but creating a comprehensive, predictive model of the cell will require a different approach."

The Allen Cell Observatory's goal is to produce a dynamic, visual database and animated models of cell parts in action. Such models could shed light on the processes by which genetic information is translated into cellular functions, and reveal what goes wrong in a diseased cell. That, in turn, could help researchers predict which therapies will work best to counter diseases, or perhaps head off the disease in the first place.

Allen's latest philanthropic venture was unveiled Monday at the American Society for Cell Biology's annual meeting in Philadelphia. It follows up on plans that the co-founder of Microsoft has had in mind for years.

"It's the right time to start a big initiative in cell biology: understanding how cells work, understanding the detailed things that happen inside cells, which is behind cancer and Alzheimer's and all those things," Allen told NBC News last year.

Software billionaire Paul Allen's latest philanthropic project is a $100 million commitment to create the Allen Institute for Cell Science.

Paul Allen's net worth is estimated at more than $17 billion. Over the past 15 years, he has contributed hundreds of millions of dollars to scientific projects including the Allen Telescope Array, the Allen Institute for Brain Science and the Allen Institute for Artificial Intelligence. Last month, he said he would contribute $100 million to the global fight against the Ebola virus. (Allen also owns somewhat less-scientific ventures, such as the Seattle Seahawks and the Portland Trail Blazers.)

The cell science institute will be housed in the seven-story Allen Institute headquarters building that is currently under construction in Seattle's South Lake Union neighborhood. The building is scheduled for completion in the fall of 2015, and will also house the Allen Brain Institute.

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Billionaire Paul Allen Pledges Millions for Cell Science

Irvine, California (PRWEB) December 08, 2014

The Ageless Derma skin care company has added a moisturizing product to their line that provides continuous hydration to skin throughout the day. The Swiss Apple Stem Cell Oil-Free Continuous Moisturizer uses rare Swiss apple stem cells in combination with other natural substances to aid in skins retention of moisture for a lessening of fine lines and a silky, more comfortable feeling.

The Swiss Apple Stem Cell Oil-Free Continuous Moisturizer contains stem cells from the exotic Malus Domestica, a rare apple from Switzerland known for its long shelf life and its ability to stay fresh without shriveling. This apple species had a flavor that consumers found too acidic, making farmers reluctant to grow it. The Malus Domestica, however, was discovered to have interesting scientific advantages due to its ability to live a long, healthy life without the usual shriveling that accompanies fruit as it ages. The same idea has been transferred to Ageless Dermas latest moisturizer with its incorporation of these stem cell extracts for a renewed and rejuvenated facial complexion. The stem cells help with not only apple longevity, but also with repairing human skin cells. This results in the ultimate reduction of fine lines and wrinkles with regular use.

Other ingredients are added to the Swiss Apple Stem Cell Oil-Free Continuous Moisturizer to make this moisturizer a workhorse of anti-aging and hydrating skin renewal. Ceramides and essential fatty acids account for maximum skin hydration and strengthening of the skin barrier function. Capric Triglycerides silken skin, glycerin keeps moisturization and hydration in balance, and Ceramides 3, 611, and 1 (all lipids) stop moisture from escaping and hold the skin barrier intact. Swiss Apple Stem Cell Oil-Free Continuous Moisturizer also has sodium hyaluronate to attract and keep moisture in. The hyaluronate also aids in blood microcirculation and the smoothing of wrinkles.

The developers at Ageless Derma Skin Care know they are making something extraordinary happen. Their line of physician-grade skin care products incorporates an important philosophy: supporting overall skin health by delivering the most cutting-edge biotechnology and pure, natural ingredients to all of the skin's layers. This attitude continues to resonate to this day with the companys founder, Dr. Farid Mostamand, who nearly ten years ago began his journey to deliver the best skin care alternatives for people who want to have healthy and beautiful looking skin at any age. About this latest Ageless Derma product, Dr. Mostamand says, The Swiss Apple Stem Cell Oil-Free Continuous Moisturizer is a multi-beneficial product that protects skin and works to smooth lines and wrinkles as it keeps moisture in, working throughout the entire day. Without the correct distribution of moisture, skin becomes dry and susceptible to wrinkling. This product is oil-free and can be used for any skin type.

Ageless Derma products are formulated in FDA-approved Labs. All ingredients are inspired by nature and enhanced by science. Ageless Derma products do not contain parabens or any other harsh additives, and they are never tested on animals. The company has developed five unique lines of products to address any skin type or condition.

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Ageless Derma Launches Its Latest Moisturizing Product Featuring Exotic Apple Stem Cells

Experience a New Dimension in Skin Care

The RG-Cell is the latest, breakthrough, anti-aging, skin care cosmaceutical to hit the market. It features a unique proprietary blend of stem cell activators programmed to protect your skin and visibly fight aging at the cellular level.

Scientists have shown that reactivating your dormant stem cells is the most effective process for skin rejuvenation and regeneration. This process stimulates fibroblast production of collagen, increasing skin firmness and elasticity, while reducing the appearance of fine lines and wrinkles for a smoother, silkier, vibrant and younger looking skin.

The Mayo Clinic defines Stem cells are the body's raw materials: They are cells from which all other cells with specialized functions are generated. Under the right conditions in the body or in a laboratory, stem cells divide to form more cells, called daughter cells. These daughter cells either become new stem cells (self-renewal) or become specialized cells (differentiation) with a more specific function, such as blood cells, brain cells, skin cells or heart muscle or bone. Stem cells are unique no other cell in the body has the natural ability to generate new cell types.

They can divide (through mitosis where they split into 2 separate but identical sets with 2 separate nuclei) or differentiate into diverse and specific cell types and can self-renew to produce more stem cells. In mammals, there are two broad types of stem cells:

Many specialized cells, such as in the skin, or blood, have a lifespan of only a few days. For these tissues to function, a steady replenishment of specialized cells is indispensable.

First, they are able to differentiate into all the different cell types that make up their respective tissue a property called pluripotency.

Second, they need to renew themselves in order to be able to supply new specialized tissue cells throughout life.

Skin is an essential tissue in our bodies. It is our bodys largest organ. Our skin protects us from infection, irritation and dehydration, and allows us to feel many different things, such as pressure, stress or heat. Our skin has to be constantly renewed throughout our lives and relies on a whole host of different stem cells to keep it in good shape.

Stem cells (SCs) residing in the epidermis and hair follicle ensure the maintenance of adult skin homeostasis and hair regeneration, but they also participate in the repair of the epidermis after injuries.

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Skin Care Treatment with RG-Cell Stem Cell Support Serum

Released: 1-Dec-2014 1:00 PM EST Embargo expired: 3-Dec-2014 5:00 AM EST Source Newsroom: McMaster University Contact Information

Available for logged-in reporters only

Newswise Hamilton, ON (Dec. 3, 2014) Scientists at McMaster University have discovered that human stem cells made from adult donor cells remember where they came from and thats what they prefer to become again.

This means the type of cell obtained from an individual patient to make pluripotent stem cells, determines what can be best done with them. For example, to repair the lung of a patient with lung disease, it is best to start off with a lung cell to make the therapeutic stem cells to treat the disease, or a breast cell for the regeneration of tissue for breast cancer patients.

Pluripotency is the ability stem cells have to turn into any one of the 226 cell types that make up the human body.The work challenges the previously accepted thought that any pluripotent human stem cell could be used to similarly to generate the same amount of mature tissue cells.

This finding, published today in the prestigious science journal Nature Communications, will be used to further drug development at McMaster, and potentially improve transplants using human stem cell sources.

The study was led by Mick Bhatia, director of the McMaster Stem Cell and Cancer Research Institute. He holds the Canada Research Chair in Human Stem Cell Biology and he is a professor in the Department of Biochemistry and Biomedical Sciences of the Michael G. DeGroote School of Medicine.

Its like the stem cell we make wants to become a doctor like its grandpa or an artist like its great-grandma, said Bhatia.

Weve shown that human induced pluripotent stem cells, called iPSCs, have a memory that is engraved at the molecular/genetic level of the cell type used to make them, which increases their ability to differentiate to the parent tissue type after being put in various stem cell states.

So, not all human iPSCs are made equal, Bhatia added. Moving forward, this means that iPSC generation from a specific tissue requiring regeneration is a better approach for future cellular therapies. Besides being faster and more cost-efficient in the development of stem cell therapy treatments, this provides a new opportunity for use of iPSCs in disease modeling and personalized drug discovery that was not appreciated before.

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Not All Induced Pluripotent Stem Cells Are Made Equal

PUBLIC RELEASE DATE:

3-Dec-2014

Contact: Veronica McGuire vmcguir@mcmaster.ca 90-552-591-402-2169 McMaster University @mcmasteru

Hamilton, ON (Dec. 3, 2014) - Scientists at McMaster University have discovered that human stem cells made from adult donor cells "remember" where they came from and that's what they prefer to become again.

This means the type of cell obtained from an individual patient to make pluripotent stem cells, determines what can be best done with them. For example, to repair the lung of a patient with lung disease, it is best to start off with a lung cell to make the therapeutic stem cells to treat the disease, or a breast cell for the regeneration of tissue for breast cancer patients.

Pluripotency is the ability stem cells have to turn into any one of the 226 cell types that make up the human body.The work challenges the previously accepted thought that any pluripotent human stem cell could be used to similarly to generate the same amount of mature tissue cells.

This finding, published today in the prestigious science journal Nature Communications, will be used to further drug development at McMaster, and potentially improve transplants using human stem cell sources.

The study was led by Mick Bhatia, director of the McMaster Stem Cell and Cancer Research Institute. He holds the Canada Research Chair in Human Stem Cell Biology and he is a professor in the Department of Biochemistry and Biomedical Sciences of the Michael G. DeGroote School of Medicine.

"It's like the stem cell we make wants to become a doctor like its grandpa or an artist like its great-grandma," said Bhatia.

"We've shown that human induced pluripotent stem cells, called iPSCs, have a memory that is engraved at the molecular/genetic level of the cell type used to make them, which increases their ability to differentiate to the parent tissue type after being put in various stem cell states.

Continued here:
Not all induced pluripotent stem cells are made equal: McMaster researchers

NEWS

1200+ scientists, patient advocates from 40 countries in town for summit

Posted December 02, 2014, 6:04 PM Updated December 02, 2014, 6:33 PM

SAN ANTONIO - More than a thousand scientists, industry leaders and patient advocates from 40 countries are headed to San Antonio for the World Stem Cell Summit.

Organizers are calling it the center of the universe when it comes to stem cells and regenerative medicine.

On Tuesday the summit kicked off with Public Education Day, where some of the smartest scientists in the field broke the topic down into bite-sized pieces.

"To be able to replenish our cells that die within a tissue on a daily basis, in order for us to be able to heal wounds, we have to have stem cells," said Elaine Fuchs, an investigator for the Howard Hughes Medical Institute.

She started her research in the field in the 1970s with work on skin stem cells, and said she was fascinated with creating skin in a petri dish that could then be used for burn therapy.

Fuchs spoke at Public Education Day about the most basic biology of stem cells and said that knowledge is leading to a new world in medicine.

"The biology of stem cells is gong to be and is being extremely valuable in terms of developing new therapies and coming up with new drugs to treat various different devastating diseases," Fuchs said.

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World Stem Cell Summit kicks off in SA with Public Education Day

This active ingredient won the prize in European Innovation Best Active Ingredient in 2008. It is a revolutionary technology designed to protect human skin stem cells with the help of stem cells from a rare Swiss apple. The clinical trials conducted by the company who discovered this ingredient showed that 100% of the participants saw a reduction in fine lines and wrinkles after using a solution containing 2% PhytoCellTech Malus Domestica.

According to the Bible, Adam bit into an apple (coaxed on by us femme fatales) and deprived Earth of Heaven...was he attracted by the delicious taste or did he already know of the amazing youth-boosting properties of this fruit?

PhytoCellTec Malus Domestica is an award-winning patented liposomal preparation, so containing tiny bubbles made out of the same material as cell membranes, based on the stem cells of a rare Swiss apple called Uttwiler Sptlauber that derives from a seedling planted in the middle of the18th century. Uttwiler Sptlauber is an endangered apple variety that is well-known for its ability to be stored for long periods without shrivelling and thus its longevity potential. The apples are rich in phytonutrients, proteins and long-living cells. A novel technology has now been developed enabling the cultivation of rare and endangered species like Uttwiler Sptlauber. Thanks to this technology, plant stem cells can be obtained and incorporated into skin care products to enhance the longevity of skin cells. Not only does it protect the skins own stem cells but has been shown to have excellent age-delaying and anti-wrinkle properties, and is currently one of the most pioneering and exciting ingredients in skin care.

Stem Cells and Longevity

Longevity is related to specific cells called stem cells which have a unique growth characteristic. These cells can make identical copies of themselves as well as differentiate (in other words, split) to become separate, specialised cells. Two basic types of stem cells are present in the human body:

Embryonic stem cells found in blastocysts (structures found in the human pre-embryonic stage) can grow and differentiate into one of the more than 220 different cell types which make up the human body;

Adult stem cells located in some adult tissues can only differentiate into their own or related cell types. These cells act as a repair system for the body but also maintain the normal turnover of regenerative organs such as blood, skin or intestinal tissues.

Research on Stem Cells and Applications

Currently in medicine, adult stem cells are already used particularly in transplant medicine to treat leukemia and severe burns. In the cosmetic field, scientists are focusing their research on adult stem cells located in the skin. They are studying the potential of this type of cells, their functioning and aging. This research is helping us understand how to protect skin stem cells.

Stem Cells in the Human Skin

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Swiss Apple Stem Cells for perfect skin. What do plant ...

New research has found evidence that stem cells could be used to correct genetic defects in skin and to treat certain rare diseases.

Three separate studies by scientists in the US, Europe and Japan have raised hopes that the methods could be used to develop treatments for a range of problems, including epidermolysis bullosa.

It is a disorder wheresufferers are born with extensive blistering and patches of missing skin.

They areleft with extremely fragile skin for all of their lives.

In the first study, the researchers used Induced Pluripotent Stem Cells (iPSCs) - adult cells that are reprogrammed to an embryonic stem cell-like state.

The scientists took diseased cells from three adult patients withepidermolysis bullosa.

The researchers converted the cells into iPSCs and used specialist tools to edit and fix the mutation in the genetic code responsible for defective collagen protein production, which causes the condition.

They then grew pieces of human skin that produced the correct collagen, and grafted them into mice where they lasted for three weeks.

It i's hoped the risk of rejection in humans will be minimal because the skin is made from the patient's own cells.

A second study confirmed these findings in the lab, showing that it is possible to genetically correct iPSCs from mice with epidermolysis bullosa and use the repaired cells to heal blistered skin.

Continued here:
Scientists use stem cells to correct skin defects

Irvine, California (PRWEB) November 27, 2014

The Ageless Derma skin care company has just released their latest development in the form of a facial mask that exfoliates skin with ingredients such as apple stem cells to renew the complexion and correct texture and tone. The companys Swiss Apple Stem Cell Mask incorporates the cells of a long-living rare apple with other revitalizing ingredients from nature to result in a gentle mask that is effective and calming.

The Swiss apple, Malus Domestica, has its beginnings that go as far back as 18th century Switzerland. Ageless Derma recognized the importance of this plants stem cell extract for its ability to keep the fruit fresh for extended periods of time without wrinkling or shriveling. The Swiss Apple Stem Cell Mask contains the scientific advances that come from the cultivation of these stem cells, having incorporated it into a powerful and effective facial mask to rejuvenate skin and keep wrinkles at bay.

The Swiss Apple Stem Cell Mask contains other natural ingredients that work together to keep skin at its purest and return youthful life to the complexion. Kaolin Clay from the earth absorbs toxins that can enter the skins surface due to environmental pollutants in the air. The clay helps draw out grime and purify skin. Sweet Almond Oil nourishes skin, and adds much needed moisture and smoothness. Safflower Oil improves the texture of skin; especially skin that has become roughened with time and sun exposure. The Safflower Oil in Swiss Apple Stem Cell Mask also locks in moisture and tones skin for a flawless and radiant complexion.

Ageless Derma added fruit extracts to the Swiss Apple Stem Cell Mask for added health and radiance. Pumpkin Fruit Ferment, Pineapple Enzyme, and Papaya Enzyme make this mask luscious and plush. Age-defying antioxidants are also included, with Green Tea Extract and Aloe Leaf Extract added for soothing and fighting free radicals.

The developers at Ageless Derma Skin Care know they are making something extraordinary happen. Their line of physician-grade skin care items incorporates an important philosophy: promoting overall skin health by delivering the most cutting-edge biotechnology and pure, natural ingredients to all of the skin's layers. This attitude continues to resonate to this day with the companys founder, Dr. Farid Mostamand, who nearly a decade ago began his journey to deliver the best skin care alternatives for people who want to have healthy and beautiful looking skin at any age. About this latest Ageless Derma product, Dr. Mostamand says, This natural enzymatic Swiss Apple Stem Cell Mask gently exfoliates dead skin cells that are blocking new cell turnover for a renewed and radiant complexion. This is accomplished without the use of unnatural chemicals that can harm your skins delicate balance.

Ageless Derma products are formulated in FDA-approved Labs. All ingredients are inspired by nature and enhanced by science. Ageless Derma products do not contain parabens or any other harsh additives, and they are never tested on animals. The company has developed five unique lines of products to address any skin type or condition.

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Ageless Derma Introduces Their Latest Innovation: Swiss Apple Stem Cell Mask

27.11.2014 - (idw) IMBA - Institut fr Molekulare Biotechnologie der sterreichischen Akademie der Wissenschaften GmbH

Scientists at IMBA Institute of Molecular Biotechnology of the Austrian Academy of Sciences in Vienna have made a major advancement towards a future therapy for butterfly children. A treatment with fibroblasts generated from induced pluripotent stem cells has been highly successful in mice. The next step is to establish this method in humans. Butterfly children suffer from Epidermolysis Bullosa (EB), a debilitating skin disease. It is caused by a genetic defect that leads to a deficiency or complete lack of various structural proteins. In one particularly severe form, the protein collagen 7 is either missing or present only in insufficient amounts. If that bond is missing, the skin forms blisters or tears at the slightest mechanical pressure, leading to wounds and inflammation that require extensive treatment with creams and bandages. Often these constant lesions also lead to aggressive forms of skin cancer.

Presently there is no cure for this disease. But there are promising approaches that could lead to successful treatments in the future. One of them is a method called fibroblast injection. In this procedure, fibroblasts are injected between the layers of the skin, where they can produce the necessary collagen 7.

Researchers at IMBA under the leadership of Arabella Meixner have now been successful in developing this method to treat mice affected by EB. The individual steps of this treatment have been worked out and carefully tested in many years of laboratory work, and the results have now been published in the scientific journal Science Translational Medicine.

First the scientists returned skin cells of the diseased mice to the stem cell stage and then repaired the genetic defect, the root cause of the disease. Then the researchers transformed stem cells back into fibroblasts.

Before the repaired fibroblasts could be reintroduced into the organism, measures to prevent inflammation or rejection were necessary. In this study the researchers conducted a type of toxicity test, and the results were very promising. After several months of observation, no adverse immune reactions occurred, and the risk of skin cancer did not increase. That is an important consideration because butterfly children already have a greatly increased risk of skin cancer.

The next step is to establish this skin stem cell treatment in humans. To achieve that, the IMBA scientists intend to look for partners with clinical experience. For severe forms of Epidermolysis Bullosa, a systemic application needs to be developed to spread the cells throughout the entire body via the bloodstream to reach epithelial tissues that are more difficult to access, for example the mucous membranes in the mouth or bowels. Often in butterfly children with milder forms of the disease, only certain areas of the skin are affected. The skin stem cell therapy with local injections successfully tested on mice could lead to a valuable treatment method in the very near future.

The project conducted by IMBA scientists was initiated by the patient organization DEBRA Austria, and has had the financial support of the association and of other generous supporters since 2009. DEBRA's mission is to ensure that butterfly children receive competent specialized medical care and to promote research into options to relieve and cure EB. Further thanks also go to our funding and cooperation partners sterreichische Lotterien and FK Austria Wien.

Original publication: Wenzel et. al., iPSC-based cell therapy for Recessive Dystrophic Epidermolysis Bullosa. Science Translational Medicine. 2014.

Scientific Contact: Dr. Arabella Meixner, Research Lead Tel. +43 664 2018084 arabella.meixner@imba.oeaw.ac.at Weitere Informationen:http://www.imba.oeaw.ac.at

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Tremendous progress in the development of skin stem cell treatments for butterfly children

A team led by scientists from The Scripps Research Institute (TSRI) has found a simple method to convert human skin cells into the specialized neurons that detect pain, itch, touch and other bodily sensations. These neurons are also affected by spinal cord injury and involved in Friedreich's ataxia, a devastating and currently incurable neurodegenerative disease that largely strikes children.

The discovery allows this broad class of human neurons and their sensory mechanisms to be studied relatively easily in the laboratory. The "induced sensory neurons" generated by this method should also be useful in the testing of potential new therapies for pain, itch and related conditions.

"Following on the work of TSRI Professor Ardem Patapoutian, who has identified many of the genes that endow these neurons with selective responses to temperature, pain and pressure, we have found a way to produce induced sensory neurons from humans where these genes can be expressed in their 'normal' cellular environment," said Associate Professor Kristin K. Baldwin, an investigator in TSRI's Dorris Neuroscience Center. "This method is rapid, robust and scalable. Therefore we hope that these induced sensory neurons will allow our group and others to identify new compounds that block pain and itch and to better understand and treat neurodegenerative disease and spinal cord injury."

The report by Baldwin's team appears as an advance online publication in Nature Neuroscience on November 24, 2014.

In Search of a Better Model

The neurons that can be made with the new technique normally reside in clusters called dorsal root ganglia (DRG) along the outer spine. DRG sensory neurons extend their nerve fibers into the skin, muscle and joints all over the body, where they variously detect gentle touch, painful touch, heat, cold, wounds and inflammation, itch-inducing substances, chemical irritants, vibrations, the fullness of the bladder and colon, and even information about how the body and its limbs are positioned. Recently these neurons have also been linked to aging and to autoimmune disease.

Because of the difficulties involved in harvesting and culturing adult human neurons, most research on DRG neurons has been done in mice. But mice are of limited use in understanding the human version of this broad "somatosensory" system.

"Mouse models don't represent the full diversity of the human response," said Joel W. Blanchard, a PhD candidate in the Baldwin laboratory who was co-lead author of the study with Research Associate Kevin T. Eade.

A New Identity

For the new study, the team used a cell-reprogramming technique (similar to those used to reprogram skin cells into stem cells) to generate human DRG-type sensory neurons from ordinary skin cells called fibroblasts.

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Pain and itch in a dish: Scientists convert human skin cells into sensory neurons

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Researchers find stem cells that help nails regenerate

November 25, 2014

Chuck Bednar for redOrbit.com Your Universe Online

Two different teams of researchers, one led by scientists from The Scripps Research Institute (TSRI) and the other involving members of the Harvard Stem Cell Institute (HSCI) have discovered ways to create the neurons that detect pain, itch and other sensations in laboratory conditions out of human and mouse skin cells.

The TSRI study, which was published online Monday in the journal Nature Neuroscience, used what the authors referred to as a simple technique to create neurons that normally reside in clusters called dorsal root ganglia (DRG) along the outer spine. Those neurons are often affected by spinal cord injuries and a neurodegenerative condition known as Friedreichs ataxia.

According to the researchers, DRG sensory neurons extend their nerve fibers into skin, muscle and joints located throughout the body. The neurons are capable of alternately detecting gentle touch, painful contact, heat, cold, wounds, inflammation, chemical irritants, itch-inducing agents and fullness of the bowels and bladder. They also relay information about the position of the body and limbs, and have been linked to aging and autoimmune disease.

Due to the difficulties involved in culturing adult human neurons, most research relating to DRG neurons has been done in mice. However, the rodents are of limited use in understanding the human version of this somatosensory system, TSRI explained. The new discovery will allow this type of human neurons and their associated sensory mechanisms to be studied with relative ease in laboratory conditions, according to the study authors.

We have found a way to produce induced sensory neurons from humans where these genes can be expressed in their normal cellular environment, associate professor Kristin K. Baldwin, an investigator in TSRIs Dorris Neuroscience Center, said in a statement. This method is rapid, robust and scalable. Therefore we hope that these induced sensory neurons will allow our group and others to identify new compounds that block pain and itch and to better understand and treat neurodegenerative disease and spinal cord injury.

Similarly, the HSCI-led study, which included experts from Boston Childrens Hospital (BCH) and Harvards Department of Stem Cell and Regenerative Biology (HSCRB), was able to successfully convert mouse and human skin cells into pain-sensing neurons that responded to several different types of stimuli responsible for causing both acute and inflammatory pain.

The authors of this study, which also appeared in Wednesdays online edition of Nature Neuroscience, said that their research could help scientists better understand the different types of pain that we experience, as well as better identify why people respond to pain in different ways and why some individuals are more or less likely to develop chronic pain. It could also result in the development of improved pain-relieving medications.

The six-year project resulted in the creation of neuronal pain receptors that respond to both the types of intense stimuli triggered by a physical injury, and the more subtle stimuli triggered by inflammation which results in pain tenderness. The researchers report that the fact the neurons can respond to both the gross and fine forms of stimulation which produce separate types of pain in humans confirm that they are functionally normal.

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Pain-Sensing Neurons Created From Human, Mouse Skin Cells

Previous studieshaveunsuccessfullytried to producenerve cells from embryonic stem cells For the recent study, a team of USresearchers used adult tissue instead They were able to reprogram ordinary skin cells into induced stem cells Scientistsat Harvard Medical School in Massachusetts used a cocktail of proteins called transcription factors that control the activity of genes Study could help reveal the origins of pain and develop better drugs

By Sarah Griffiths for MailOnline

Published: 13:04 EST, 24 November 2014 | Updated: 13:16 EST, 24 November 2014

Pain is a complex and unpleasant sensation, which some people feel more acutely than others - and its origins remain largely a mystery.

Now, scientists have created pain in a dish by converting skin cells into sensitive neurons in a bid to learn more about these sensations.

The lab-created nerve cells respond to a range of different kinds of pain stimulation, including physical injury, chronic inflammation and cancer chemotherapy.

Scientists have created pain in a dish by converting skin cells into sensitive neurons (illustrated) in a bid to learn more about its origins.In the future, the research could be used to develop better pain-relieving drugs

And in the future, the custom-made neurons could be used to investigate the origins of pain and develop better pain-relieving drugs.

The work follows years of unsuccessful attempts to produce nerve cells from embryonic stem cells, which are immature blank slate cells with the potential to become any tissue in the body.

A nociceptor is a receptor of a nerve cell that responds to potentially damaging stimuli by sending signals to the spinal cord and brain.

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Nerve cells 'grown' in a lab could reveal more about how injury affects the body